Comprehensive Evaluation of Health Status of Traditional Chinese Medicine Constitution based on Improved Radar Chart / 中医杂志

ObjectiveTo present the health status of traditional Chinese medicine （TCM） constitutions more intuitively and comprehensively based on improved radar chart. MethodsParticipants who completed a 26-week comprehensive intervention based on TCM constitution from February 2013 to January 2014 in Zhuhai branch of Guangdong Provincial Hospital of Chinese Medicine were included in the study. They were divided into groups according to gender and age， i.e. young， middle-aged， and elderly male and female groups. TCM constitution scale and health survey short form （SF-36） were used to evaluate the 9 basic TCM constitution types and quality of life at three time points， including pre-intervention （T1）， at 13-week intervention （T2）， and at 26-week intervention （T3）. The improved radar charts were drawn to visually present the comprehensive evaluation results on the health status of 9 TCM constitutions， and graphic features （area S value， perimeter L value） were extracted to construct a comprehensive health index for TCM constitutions （H value）. Spearman correlation analysis was used to analyze the correlation between H value and SF-36 total score. ResultsAmong the included 509 participants， there were 45 elderly male， 76 elderly female， 60 middle-aged male， 140 middle-aged female， 53 young male and 135 young female. The radar charts for comprehensive evaluation of TCM constitution health status showed that the total areas for all groups increased at T3 compared to T1， with the most significant increase in the young population. In the middle-aged population， the fan-shaped areas of certain constitutions decreased at T2 than T1. At T3， the radar chart shapes for females were more balanced than males in the same age group. By calculating the features of function graphs， it was found that the S， L， and H values for the elderly population were relatively higher than those for the middle-aged and young population with the same gender， and the young population increased by highest ratio. The values measured at T3 compared to T1 showed average increase of 26% for S value （11% for the middle-aged and 14% for the elderly）， 22% for L value （10% for the middle-aged and the elderly each）， and 22% for H value （10% for the middle-aged and 9% for the elderly）. The female had lower S and L values， as well as higher H value than the male of the same age group measured at T3. The correlation coefficient between the H value of all participants and the total SF-36 score was 0.662 （P＜0.01）. ConclusionThe comprehensive evaluation model for the health status of TCM constitution based on the improved radar chart constructed in this study can present the health status of TCM constitutions and intervention effectiveness more comprehensively and intuitively. It is suggested to regulate the constitution in pursuit of the dynamic balance of the constitution health status， as well as consider the parts from the whole， and put focus on the balance of nine TCM constitutions.

The compound cell model-based evaluation for idiosyncratic liver injury of Cis-SG and Trans-SG / 药学学报

In this study, a composite cell model for evaluation of idiosyncratic drug-induced liver injury (IDILI) was established in vitro from the perspective of immune inflammation. And this model was used to evaluate the risk of IDILI for 2,3,5,4'-tetrahydroxy-cis-stilbene-2-O-β-glucoside (Cis-SG) and 2,3,5,4'-tetrahydroxy-trans-stilbene-2-O-β-glucoside (Trans-SG). To determine the low, medium, and high dosage of Cis-SG and Trans-SG, CellTiter-Glo® 3D Cell Viability Assay was used to detect the effects of Cis-SG and Trans-SG on cell viability of HepG2 cells in three dimensional (3D) culture, and MTT assay was used to detect the effects of Cis-SG and Trans-SG on cell viability of THP-1 derived macrophages. THP-1 derived macrophages were incubated by Cis-SG and Trans-SG directly or supernatants from HepG2 cells incubated with them. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of interleukin-1β (IL-1β) in the supernatants of the THP-1 derived macrophages. Western blot and reverse transcription-polymerase chain reaction (RT-PCR) were used to determine the expression of apoptosis-associated speck-like protein (ASC), Nod-like receptor protein 3 (NLRP3), cysteinyl aspartate specific proteinase-1 (caspase-1), and IL-1β in THP-1 derived macrophages. The results showed that there was no effect on the secretion of IL-1β in THP-1 derived macrophages incubated by Cis-SG and Trans-SG directly. However, the secretion of IL-1β, the protein and mRNA expression of ASC, NLRP3, caspase-1, and IL-1β significantly increased in THP-1 derived macrophages incubated by supernatants from HepG2 cells incubated with 1, 5, and 25 μmol·L-1 Cis-SG or 25 μmol·L-1 Trans-SG. In summary, the composite cell model for evaluation of IDILI established in vitro has been successfully applied in testing Cis-SG and Trans-SG. This composite cell model is helpful to evaluate and screen drugs with IDILI risk in vitro preliminarily, which provides methods for predicting and solving the idiosyncratic liver toxicity of drugs.

Una vía para la predicción de una laringoscopia anatómicamente difícil / A way to predict an anatomically difficult laryngoscopy / Uma maneira de prever uma laringoscopia anatomicamente difícil

RESUMEN Introducción: En la valoración de dificultad para realizar la laringoscopia convencional no se realza la integración necesaria de aspectos clínicos esenciales relacionados con el control respiratorio. Objetivo: Validar un modelo de predicción de una laringoscopia anatómicamente difícil en el paciente que requiere de intubación orotraqueal. Método: Se realizó un estudio analítico en una población de 17 966 pacientes con necesidad de laringoscopia directa para una intubación orotraqueal con fines quirúrgicos en el Hospital General Docente "Dr. Agostinho Neto" de Guantánamo entre el 2015 y el 2018. Se determinó por muestreo aleatorizado una muestra de 17 068 pacientes. Se estudiaron las siguientes variables: estigma periférico para laringoscopia difícil, laringoscopia difícil pos-inducción anestésico, evaluación laringoscópica según Cormack-Lehane, valor diagnóstico del modelo de evaluación predictiva para laringoscopia. Resultados: Los altos grados en la clasificación de aspectos clínicos predictivos y la coexistencia con la alteración morfológica de la epiglotis fueron los marcadores más asociados con la probabilidad de laringoscopia anatómicamente difícil. Con la integración de cuatros aspectos clínicos esenciales se identificó el grado de dificultad probable para visualizar las cuerdas vocales. Conclusiones: Se diseñó un modelo que posibilitó la predicción de una laringoscopia anatómicamente difícil, cuya validación certificó su viabilidad para aplicarlo en la práctica médica.

ABSTRACT Introduction: In assessing the difficulty of performing conventional laryngoscopy, the necessary integration of essential clinical aspects related to respiratory control is not enhanced. Objective: To validate a prediction model of an anatomically difficult laryngoscopy in the patient that requires orotracheal intubation. Method: An analytical study was carried out in a population of 17,966 patients in need of direct laryngoscopy for an orotracheal intubation for surgical purposes at the General Teaching Hospital "Dr. Agostinho Neto" from Guantanamo between 2015 and 2018. A sample of 17,068 patients was determined by randomized sampling. The following variables were studied: peripheral stigma for difficult laryngoscopy, difficult laryngoscopy after anesthetic induction, laryngoscopic evaluation according to Cormack-Lehane, diagnostic value of the predictive evaluation model for laryngoscopy. Results: The high degrees in the classification of predictive clinical aspects and the coexistence with the morphological alteration of the epiglottis were the markers most associated with the probability of anatomically difficult laryngoscopy. With the integration of four essential clinical aspects, the degree of probable difficulty in visualizing the vocal cords was identified. Conclusions: A model was designed that allowed the prediction of an anatomically difficult laryngoscopy, whose validation certified its feasibility to apply it in medical practice.

RESUMO Introdução: Na avaliação da dificuldade na realização da laringoscopia convencional, a integração necessária dos aspectos clínicos essenciais relacionados ao controle respiratório não é aprimorada. Objetivo: Validar um modelo de previsão de uma laringoscopia anatomicamente difícil no paciente que necessita de intubação orotraqueal. Método: Foi realizado um estudo analítico em uma população de 17.966 pacientes com necessidade de laringoscopia direta para intubação orotraqueal para fins cirúrgicos no Hospital Geral de Ensino "Dr. Agostinho Neto" de Guantánamo entre 2015 e 2018. Uma amostra de 17.068 pacientes foi determinada por amostragem aleatória. Foram estudadas as seguintes variáveis: estigma periférico para laringoscopia difícil, laringoscopia difícil após indução anestésica, avaliação laringoscópica segundo Cormack-Lehane, valor diagnóstico do modelo de avaliação preditiva para laringoscopia. Resultados: Os altos graus na classificação dos aspectos clínicos preditivos e a coexistência com a alteração morfológica da epiglote foram os marcadores mais associados à probabilidade de laringoscopia anatomicamente difícil. Com a integração de quatro aspectos clínicos essenciais, foi identificado o grau de provável dificuldade na visualização das cordas vocais. Conclusões: Foi elaborado um modelo que permitia prever uma laringoscopia anatomicamente difícil, cuja validação atestava sua viabilidade de aplicá-la na prática médica.

Advances in Evaluation Methods and Models on Angiogenesis / 中国药学杂志

Angiogenesis, the process of shaping new vessels from existing or posteriorvessels. It occurs mainly after birth and is the basis for the formation of many vascular structures and participates ina variety of physiological and pathological processes.Due to the particularity of the research objects and the diversity of the species, the experimental results obtained from one model may not be confirmed on the other model. At present,the comprehensive evaluation of angiogenesis requires mutual verification of different model results. Herein, this review mainly summarizes the literatures on the evaluation of angiogenesis activity within recent ten years. From three parallel dimensions of cell, tissue, and animal,the up-to-date evaluation methods and angiogenesis models with wide recognitions are reviewed, in order to provide the reference for related drug research and development.

Establishment of Antibiotics Use Rationality Evaluation Model in Patients Underwent Type Ⅰ Incision Surgery by Means of Machine Learning Method / 中国药房

OBJECTIVE： To establish antibiotics use rationality evaluation model in type Ⅰ incision surgery patients， and to provide reference for prescription review of clinical pharmacists. METHODS： Totally 432 inpatients underwent type Ⅰ surgical incision in a hospital from Jan. 1st- Dec. 31st， 2017 were selected as the research objects. The information of diagnosis and treatment including age， nosocomial infection， the number of kinds of antibiotics used were extracted. Based on the results of clinical pharmacists’ comments on the antibiotics use rationality in patients’ prevention and treatment， non-conditional Logistic regression and support vector machine （SVM） in machine learning method were used to convert clinical pharmacists’ comments into objective index that can be recognized by the machine learning model， using categories of antibiotics （preventive or therapeutic use） as dependent variables and the patient’s diagnosis and treatment information as independent variables. Classification and identification model was established for antibiotics use rationality in type Ⅰ incision surgery patients. Using sensitivity， specificity and Youden index as indexes， established mode was validated on the other 61 samples of type Ⅰ incision surgery patients. The rationality of antibiotics prescriptions in type Ⅰ incision surgery patients before （by manual review， Jan.-Dec. 2017） and after （Jan.-Oct. 2018） using the model were collected， and the effects of the model were evaluated. RESULTS： The sensitivity， specificity and Youden index of non-conditional Logistic regression model were 65.63％， 75.00％ and 40.63％， respectively. Main parameters of the model established by SVM included gamma 0.01， cost 10， sensitivity 92.19％， specificity 87.50％， Youden index 79.69％. The model established by SVM was better than non-conditional Logistic regression. SVM was used to validate established mode， and sensitivity， specificity and Youden index were 100％， 88.57％ and 88.57％， respectively. Compared with before using the model， the evaluation ratio increased from 69.44％ to 100％， the rate of prophylactic use of antibiotics decreased from 23.84％ to 16.43％， the rate of rational drug type selection increased from 37.86％ to 54.39％， and treatment course shortened from 5.01 days to 3.26 days after using the model. CONCLUSIONS： Established antibiotics use rationality evaluation model in typeⅠincision surgery patients by SVM in machine learning method fully covers all the patients， promotes rational use of antibiotics in typeⅠincision surgery patients， and provides a new idea for pharmacist prescription comment.

Establishment of Rational Drug Use Evaluation Model Based on 360-degree Feedback Evaluation Method and Hesitant Fuzzy Sets Theory / 中国药房

OBJECTIVE： To establish a comprehensive and multi-angle evaluation model for rational drug use in medical institutions， and to provide support for rational drug use in medical institutions. METHODS： Referring to the phenomenon of irrational drug use and its influential factors in medical institutions， rational drug use evaluation indicator system， which contained 3 evaluation subjects， 6 first-level indicators and 15 second-level indicators， was established on the basis of 360-degree Feedback Method， with hospitals at the same level， superior regulation department and patient as subjects of multi-angle evaluation. At the same time， the linguistic information of vague and uncertain evaluation given by the evaluation subject was comprehensively quantified by Hesitant Fuzzy Sets Theory. The total evaluation score of rational drug use in the evaluated medical institutions was calculated by using the score function formula of hesitant fuzzy number； the level of rational drug use in medical institutions was determined according to the score （the higher the total score， the higher the comprehensive level of rational drug use）. RESULTS： An all-round and multi-angle evaluation model of rational drug use in medical institutions was established. According to the evaluation index system based on 360-degree feedback evaluation method， the evaluation subject and weight of evaluation index were determined， the grade of evaluation index was divided， the hesitant fuzzy numbers of each index were gathered， and the first-level index was weighted. Finally， the total evaluation score of rational drug use in medical institutions was calculated； the examples were set to prove the feasibility of the model. CONCLUSIONS： The established evaluation model for rational drug use in medical institutions based on 360-degree Feedback Method and Hesitant Fuzzy Sets Theory can comprehensively and effectively evaluate the rational drug use in medical institutions， and contribute to standardizing and improving clinical drug use behavior in medical institutions.

Evaluation of Medical Humanities Course in College of Medicine Using the Context, Input, Process, and Product Evaluation Model

BACKGROUND: Seoul National University College of Medicine has provided a new educational curriculum on basis of the competency-based curriculum since 2016. The new curriculum included the medical humanities course (MHC) to potentiate humanities of medical students. The present study applied the context, input, process and product (CIPP) evaluation model to the MHC in order to confirm the feasibility of the CIPP model and to improve the MHC by questionnaire survey and analysis of teaching materials. METHODS: This study analyzed the MHC provided to the freshmen in 2016 and to the freshmen and sophomores in 2017 by the CIPP model. Firstly, evaluation criteria and indicators were developed according to the CIPP classification. The materials collected from the course were analyzed by quantitative and qualitative analysis according to the evaluation criteria. In the quantitative analysis, an independent sample t-test was performed to verify the difference in the responses between the students (n = 522) and the professors (n = 22). In addition, content analysis was conducted for qualitative evaluation. RESULTS: There were significant differences in perceptions of MHC between students and professors about the results of almost all objective survey questions through the t-test, such as score 3.64 in students and 4.48 in professors in response to the item of ‘provision of appropriate feedback.’ As a result of the content analysis, 7 categories and 20 subcategories were derived. There were the most responses to various instructional methods (students, 20%; professors, 21.5%). CONCLUSION: The CIPP evaluation model was acceptable for the MHC analysis. The first task is to raise students' awareness of the MHC in order to improve the MHC quality.

Modelo teórico de evaluación de la estrategia curricular de Medicina Natural y Tradicional de la carrera de Medicina / Theoretical Model for Assessing the Curricular Strategy of Natural and Traditional Medicine in the Medical Major

Introducción: En los últimos años se ha destacado la importancia de la evaluación en la educación médica para la mejora de la calidad educativa. Objetivo: Diseñar un modelo teórico de evaluación para la estrategia curricular de Medicina Natural y Tradicional de la carrera de Medicina. Métodos: Se realizó una investigación de desarrollo en la Facultad de Ciencias Médicas Enrique Cabrera durante el curso 2015-2016, y se utilizaron los métodos de nivel teórico: el Histórico lógico, la sistematización y la modelación, y de nivel empírico, la revisión documental y el criterio de expertos, lo que permitió evidenciar los resultados de la aplicación de la estrategia curricular de Medicina Natural y Tradicional en la formación de los estudiantes de la carrera de Medicina desde las exigencias de la Educación Médica cubana actual. Resultados: Se definieron las características y principios en el modelo propuesto, se sustentaron los fundamentos desde las ciencias de la educación médica. El modelo posee carácter sistémico, flexible, permite ser modificado en función de la solución de problemas ante las necesidades de la sociedad. Se valida teóricamente por un grupo de expertos. Conclusiones: Se diseña el modelo teórico de evaluación de la estrategia curricular de Medicina Natural y Tradicional para la formación de los estudiantes en la carrera de Medicina, posibilita identificar las relaciones que se establecen entre la evaluación curricular y la aplicación de las estrategias, en particular los resultados que se alcanzan después de la aplicación del modelo teórico que enriquecen las Ciencias de la Educación Médica(AU)

Introduction: In the last years, the importance of evaluation in medical education has been highlighted for the improvement of educational. Objective: To design a theoretical model of evaluation for the curricular strategy of Natural and Traditional Medicine of the medical major. Methods: A development research was carried out at Enrique Cabrera Medical School during the academic year 2015-2016. We used theoretical methods such as the logical-historic method, systematization and modelling, and empirical methods such as document review and experts' criterion, which allowed to demonstrate the results of the application of the curricular strategy of Natural and Traditional Medicine in the training of medial students considering the demands of Cuban current medical education. Results: We defined the characteristics and principles in the proposed model and credited the foundations as based on the medical education sciences. The model has a systemic and flexible character, can be modified depending on the solution of problems in view of the societal needs. It was validated theoretically by a group of experts. Conclusions: The theoretical model for the evaluation of the curricular strategy of Natural and Traditional Medicine for the training of students in the medical major is designed, and makes it possible to identify the relationships established between curricular evaluation and the application of the strategies, in particular the outcomes reached after the application of the theoretical model that enrich medical education sciences(AU)

Application of fuzzy comprehensive evaluation model in configuration management of medical equipment / 中国医学装备

Objective: To explore the optimal configuration methods of medical equipment of hospital so as to increase the scientific, rationality, fairness and effectiveness of decisions for equipment configuration of hospital. Methods:The applications of ultrasound imaging equipment configuration of two departments were used as example. Through a series of steps, included establishing the hierarchical structure of model, constructing the judgment matrix, consistency check, determining the weight matrix, determining the evaluation set and fuzzy evaluation matrix, and calculating the evaluation vector quantity and so on, the fuzzy comprehensive evaluation model was performed and applied in the evaluation of equipment configuration. Results: After evaluation, for the ultrasound imaging equipment configuration of department 1, the ratio of experts who thought the introduction of this equipment was very good was 82.92%, and the ratio of experts who thought it was better was 12.36%, and the ratio of experts who though it was normal was 4.72%. While for department 2, the ration of experts who though it was normal or worst was more than 50%. Conclusion:The fuzzy comprehensive evaluation model is an effective method for verifying equipment configuration. It can provide effective and quantitative assessment results for decision makers of hospital and provide reference for optimal configuration of medical equipment of hospital.

Modelo procedimental de la evaluación de las políticas públicas a través de la ludificación / Modelo procedimental na avaliação das políticas públicas por meio da gamificação / Procedural model in the evaluation of public policy through gamification

Resumen Este artículo tiene por objeto proponer un modelo procedimental diseñado para optimizar la experiencia de la ciudadanía en la toma de decisiones de las políticas públicas a través de la ludificación. Se parte de la consideración que la ludificación promueve la motivación hacia una actividad prolongando su participación dentro de un ámbito específico. En este caso, se encamina su aplicación para motivar la integración de los ciudadanos en la toma de decisiones. Para ello se contrastan tres modelos de evaluación de políticas públicas que incorporan la participación civil en tres roles diferentes: auditor, pasivo y activo. Al aplicar la metodología Delphi, los resultados de fiabilidad y consistencia interna demuestran que el modelo de participación activa tiene mayor valoración por parte de los expertos respecto a la ludificación, Como conclusión, el modelo generado abre una nueva área de conocimiento dentro de la evaluación de políticas públicas imbuida en la interactividad del contexto mediático.

Resumo Este artigo tem como objetivo propor um modelo procedimental concebido para otimizar a experiência dos cidadãos na tomada de decisões de políticas públicas por meio da gamificação. Parte-se do pressuposto de que a gamificação promove a motivação para uma atividade, aumentando a participação dentro de um âmbito específico. Nesse caso, a gamificação é utilizada para incentivar o envolvimento dos cidadãos na tomada de decisões. Com esse objetivo, comparam-se três modelos de avaliação de políticas públicas que incorporam a participação civil em três papéis diferentes: auditor, passiva e ativa. Ao aplicar a metodologia Delphi, os resultados de fidelidade e consistência interna revelam que o modelo de participação ativa é alvo de uma valoração superior por parte dos especialistas, no que se refere à gamificação. Em conclusão, o modelo gerado abre uma nova área de conhecimento no âmbito da avaliação de políticas públicas, integrada na interatividade do contexto midiático

Abstract This article proposes a procedural model designed to optimize the experience of citizens in decision making of public policies through gamification. The study assumes that gamification increases motivation and contributes to increase involvement within a specific area. In this case, gamification is used to encourage citizens' engagement in decision-making, based on three models of evaluation of public policies that incorporate civil participation in three different roles: auditor, passive and active. After applying the Delphi methodology, the results of reliability and internal consistency show that experts attribute a higher value to the model of active participation using gamification. In conclusion, the model designed opens a new area of knowledge within the evaluation of public policies, embedded in the interactivity of the media context.

Exploration and research on comprehensive evaluation model of Chinese patent medicines supply in national essential drugs / 中国中药杂志

Chinese medicine prices often have sharp rise or fall, and even the impact of short-term price rise on the quality of feed can't be ignored.On the other hand, the production of traditional Chinese medicine is strictly subject to resource constraints, so much of the industrial production due to lack of or prohibited use of resources must bestopped. Based on 203 Chinese patent medicines in the National Essential Drugs (2012 Edition), the factors that influence the supply of essential drugs of traditional Chinese medicine were analyzed and seven indicators for the supply of essential medicines were designed in this paper. According to the Chinese herbal medicine resources survey and dynamic monitoring service system data, a multi-index comprehensive evaluation model based on radar chart analysis was proposed, providing a holistic and overall evaluation of the supply situation of essential drugs of traditional Chinese medicine. The evaluation results were concise, clear and intuitive. In this paper, Jiuwei Qianghuo Wan and Ganmao Qingre Keli were taken as the examples to prove that the proposed comprehensive evaluation method is concise, clear, intuitive, credible and practical.

Research on Quality of Angelicae Sinensis Radix Based on AHP Fuzzy Comprehensive ;Evaluation Model / 中国中医药信息杂志

Objective To establish a method to evaluate quality of Angelicae Sinensis Radix based on analytic hierarchy process (AHP) fuzzy comprehensive evaluation. Methods Angelicae Sinensis Radix of different producing regions was collected. Volatile oil content, alcohol soluble extract content, as well as the composition of ferulic acid, Z-ligustilide, 3-butylphthalide and n-butylidenephthalide of Angelicae Sinensis Radix samples were separately measured. AHP was used to establish AHP analysis model to comprehensively evaluate multiple indexes of Angelicae Sinensis Radix. Results The evaluation result comparison showed that the quality of Angelicae Sinensis Radix in Min county, Weiyuan county and Zhang county were better than that of other regions, and Min county had the Angelicae Sinensis Radix with the best quality. Conclusion Evaluating Angelicae Sinensis Radix based on AHP fuzzy comprehensive evaluation model is feasible.

Evaluation on Comprehensive Scientific Research Capability of Technology Talents and Application Study Based on Big Data of Scientific and Technological Information / 医学信息学杂志

Based on the analysis of comprehensive scientific research capability of technology talents,the paper constructs the evalu ation model of comprehensive scientific research capability of technology talents,designs the evaluating indicator system based on big data of scientific and technological information,including the 5 dimensions like basic literacy,influence of scientific research output,scientific research management capability,academic potential and academic status,and studies on its application in talent introduction.

Information system success mo del-based health information system construction effect evaluation model / 中华医学图书情报杂志

Objective To establish a general health information construction effect evaluation model for overall as-sessment of health information construction effect .Methods Domestic and foreign health information construction effect evaluation models were systematically analyzed by bibliometric analysis , comparative analysis , inductive and deductive method,repectively.The classic health information construction effect models were integrated.Results The health information construction effect evaluation model was established from the technique-organization man-agement-operation supportangle .Conclusion Thehealth information construction effects include technique effect, organization mangement effect, and operation effect.Thegeneral health information construction effect evaluation model is established, which includes 7 primary indexes and 20 secondaey indexes.

3D evaluation model for drug hepatotoxicity testing on HepG2 cells and its application in drug safety evaluation / 中国中药杂志

3D in vitro toxicity testing model was developed by magnetic levitation method for culture of the human hepatoma cell line HepG2 and applied to evaluate the drug hepatotoxicity. After formation of stable 3D structure for HepG2 cells, their glycogen storage capacity under 2D and 3D culture conditions were detected by immunohistochemistry technology, and the mRNA expression levels of phase Ⅰ and Ⅱ drug metabolism enzymes, drug transporters, nuclear receptors and liver-specific marker albumin(ALB) were compared between 2D and 3D culture conditions by using RT-PCR method. Immunohistochemistry results showed that HepG2 cells had abundant glycogen storage capacity under 3D culture conditions, which was similar to human liver tissues. The mRNA expression levels of major drug metabolism enzymes, drug transporters, nuclear receptors and ALB in HepG2 cells under 3D culture conditions were up-regulated as compared with 2D culture conditions. For drug hepatotoxicity evaluation, the typical hepatotoxic drug acetaminophen(APAP), and most reported drugs Polygonum multiflorum Thunb.(Chinese name He-shou-wu) and Psoraleae corylifolia L.(Chinese name Bu-gu-zhi) were selected for single dose and repeated dose(7 d) exposure. In the repeated dose exposure test, 3D HepG2 cells showed higher sensitivity. This established 3D HepG2 cells model with magnetic levitation 3D culture techniques was more close to the human liver tissues both in morphology and functions, so it was a better 3D hepatotoxicity evaluation model.

The Effects of Clinical Education Program for Nurses in Regional Public Hospital / 보건행정학회지

BACKGROUND: The purpose of this study is to evaluate the outcomes of clinical education program for nurses in regional public hospital, utilizing the Kirkpatrick's model. METHODS: Kirkpatrik's 4-level model was applied to this study. Trainees were asked to fill out questionnaires in the middle and at the end of the program. Also administrators of excellent trainees were asked to fill out the questionnaires regarding nursing management performance after 1-2 months from the end of the training course. RESULTS: All trainees had positive reactions to the clinical education program. Not only the results of individual level (satisfaction and achievement scores, academic achievement scores, practical application rate, and educational transition factors) but also the scores of organization level (nursing management performance scores) are improved. CONCLUSION: By showing a correlation between the effectiveness factors we need to verify the relationship between these factors in a future study. In addition, development of quantitative and qualitative performance indicators are needed. To establish a long-term education system, it is required to applying the excellent trainee's successful experiences.

Exploration on feasibility of introducing bioassay method into quality evaluation of Chinese herbal medicines by studying on the correlation between antioxidant activity of Prunella vulgaris and its total phenolic acids content for example / 中国中药杂志

This paper aims to investigate the correlation between the antioxidant activity of Prunella vulgaris and its total phenolic acids content by measuring the antioxidant activity of different sources and different organs of P. vulgaris and the total contents of protocatechuic acid, protocatechuic aldehyde, caffeic acid, salviaflaside and rosmarinic acid in these samples. Using the 50% methanol extract of P. vulgaris samples as the research object, DPPH method and HPLC method were used respectively to determine the antioxidant activities and the total contents of the above-mentioned five analytes in P. vulgaris samples. 0.5 mL of 50% methanol extract of P. vulgaris reacts with 0.1 mmol•L⁻¹ DPPH ethanol solution for 60 min, then the absorbance of the reaction solution was measured at 517 nm, scavenging rate and IC₅₀ values were calculated by the absorbance and the sample concentration for evaluating the antioxidant activity. HPLC analysis was made on a C₁₈ Epic column, with acetonitrile-0.1% formic acid aqueous solution as mobile phase (gradient elution), and the detection wavelength was set at 280 nm. The correlation between the antioxidant capacity of different habitats and different organs of P. vulgaris and the total contents of five kinds of phenolic acids was analyzed by partial least squares method. The reaction dose-response range of 50% methanol extract of P. vulgaris with 0.1 mmol•L⁻¹ DPPH ethanol solution was 0.300-1.65 g•L⁻¹. When the quantities of potocatechuic acid, protocatechuic aldehyde, caffeic acid, salviaflaside and rosmarinic acid were respectively in 0.007 84-0.980, 0.011 5-1.44, 0.008 64-1.08, 0.080 0-1.00 and 0.079 8-0.998 μg range, their quantities were in good linear relationship with the corresponding peak areas. The average recovery of 5 components were 97.76%, 96.88%, 100.3%, 102.1%, 104.5%, with RSD of 1.8%, 1.6%, 1.7%, 1.6% and 1.7%, respectively. In a certain range of crude drug quantity, the antioxidant activity of each organ of P. vulgaris and total phenolic acids content inside has a good linear correlation. Therefore, in certain quality range of crude drug, DPPH bioassay combined with HPLC content determination can be used for the quality control of P. vulgaris, as is a new method for the quality control of P. vulgaris.

Application of AHP in medical equipment risk evaluation / 中国医学装备

Objective:Using analytic hierarchy process(AHP)method to construct the risk evaluation of medical equipment.Methods: Combine the professors’ suggestion, data of maintenance and quality control to several medical equipments in different hospitals. Screen six kinds of medical equipment, including infant incubator, high-frequency electrotome, defibrillator, ECG monitor, ventilator and infusion pumps.Results: Ventilator has highest risk and need to be concentrated.Conclusion: The results can guide quality control, patrol, and maintenance.

Study on the Biological Pharmaceutical Industry Cluster Competitiveness in Jiangsu Province Based on Grey Multi-index Evaluation Model / 中国药房

OBJECTIVE:To establish the evaluation index system that is suitable for biological pharmaceutical industry cluster competitiveness,and provide reference for the evaluation of biological pharmaceutical industry cluster competitiveness in Jiangsu province. METHODS:Biological pharmaceutical industry cluster enterprises in Jiangsu province were selected as research samples. Through the questionnaires,the grey multi-index evaluation method was used to determine weight set,establish evaluation sample matrix,and evaluate and analyze 3 first-level indexes(8 second-level indexes,and 23 tertiary-level indexes),such as the innova-tion knowledge spillover,innovation network and social capital,etc. RESULTS & CONCLUSIONS:The financial indicators such as investment intensity of biological pharmaceutical industry cluster in Jiangsu province were low,and there was a shortage of funds problem;new drug patents of independent intellectual property rights were insufficient,and lacked of protection of intellectu-al property rights,there was an independent innovation motivation problem;profitability indexes such as the rate of product sales were low,there was a problem of low industrialization of scientific research;social culture score in social capital level was not high,there was a problem of strengthening soft environment such as the binding biological innovation public service platform. It is suggested to strengthen the cooperation among cluster enterprises and scientific research institutions,and improve enterprise innova-tion power;improve the mechanism of biological innovation and the level of industrialization of scientific research achievements;and actively guide the various types of biological innovation public service platform to participate in the construction of the soft en-vironment of the cluster.

Evaluation of hospital diagnosis and treatment quality based on the quality-evaluation model of STEMI / 中华医院管理杂志

Objective To evaluate and compare the diagnosis and treatment quality of 15 tertiary hospitals in Beijing with the quality-evaluation model of STEMI.Methods The quality-evaluation model has been formatted with the document analysis method and expert consultation method,with the indicators weighted by analytic hierarchy process.By collecting the data of 15 hospitals,we can get the values of indicators,then synthetically evaluate and compare the diagnosis and treatment quality at these 15 hospitals with the method of WRSR.Results In the diagnosing and treating the cases of STEMI at the hospitals,gaps are found between the clinical guidelines and the tests,patient evaluation,reperfusion treatment and drug therapy,with some indicators falling even below 22％.Also,there are significant differences in the diagnosis and treatment quality among hospitals.All hospitals are consistent on the five dimensions-tests,patient evaluation,reperfusion treatment,drug therapy and prognosis.Conclusion The quality-evaluation model of STEMI can comprehensively reflect the diagnosis and treatment quality of cardiovascular medicine,and partly reflect hospital's overall management level,so as to provide operating methods in improving hospital diagnosis and treatment quality.